Ancient DNA challenges prevailing interpretations of the Pompeii plaster casts

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Highlights

We generated genome-wide data from skeletal material in Pompeii plaster casts

The data contradict prior narratives about the victims’ identities and relationships

Pompeiian individuals mainly descended from recent eastern Mediterranean immigrants
Summary
The eruption of Somma-Vesuvius in 79 CE buried several nearby Roman towns, killing the inhabitants and burying under pumice lapilli and ash deposits a unique set of civil and private buildings, monuments, sculptures, paintings, and mosaics that provide a rich picture of life in the empire. The eruption also preserved the forms of many of the dying as the ash compacted around their bodies. Although the soft tissue decayed, the outlines of the bodies remained and were recovered by excavators centuries later by filling the cavities with plaster. From skeletal material embedded in the casts, we generated genome-wide ancient DNA and strontium isotopic data to characterize the genetic relationships, sex, ancestry, and mobility of five individuals. We show that the individuals’ sexes and family relationships do not match traditional interpretations, exemplifying how modern assumptions about gendered behaviors may not be reliable lenses through which to view data from the past. For example, an adult wearing a golden bracelet with a child on their lap—often interpreted as mother and child—is genetically an adult male biologically unrelated to the child. Similarly, a pair of individuals who were thought to have died in an embrace—often interpreted as sisters—included at least one genetic male. All Pompeiians with genome-wide data consistently derive their ancestry largely from recent immigrants from the eastern Mediterranean, as has also been seen in contemporaneous ancient genomes from the city of Rome, underscoring the cosmopolitanism of the Roman Empire in this period.

https://www.cell.com/current-biology/abstract/S0960-9822(24)01361-7



DNA reveals new stories about Pompeii’s victims
‘The scientific data we provide do not always align with common assumptions.’

A new genetic analysis of 14 bodies recovered from Pompeii is casting doubt on some of the initial interpretations of their identities before the cataclysmic volcanic eruption. A team of archaeologists and geneticists used a new genomic analysis to find out more about who these individuals were. They ultimately found that their biological sexes and family relationships don’t match up with the initial interpretations. The findings are detailed in a study published November 7 in the journal Current Biology.


“This study illustrates how unreliable narratives based on limited evidence can be, often reflecting the worldview of the researchers at the time,” David Caramelli, a study co-author and archeologist at the Universita di Firenze in Italy, said in a statement.


[Related: Pompeii’s archaeological puzzles can be solved with a little help from chemistry.]


In 79 CE, the Somma-Vesuvius volcano system erupted in southern Italy. The earthquakes and violent eruption completely buried the inhabitants of the small Roman town of Pompeii. Volcanic ash covered and preserved many of the bodies. The remains of the town were initially discovered in 1599 and by the 19th Century, scientists began to uncover the remains and preserved several in plaster.


This new study probed the DNA evidence that has been taken from some of these bodies.


The team used the same strontium isotopes that were used to date the DNA samples to get a better understanding of the diversity and origins of Pompeii’s residents. The team extracted DNA from highly fragmented human skeletal remains mixed with the plaster casts that were used to preserve the bodies. They focused on 14 of 86 casts that are undergoing restoration, with the goal of using the DNA to learn as much as they could about these individuals. Their approach allowed them to accurately determine the genetic relationships, sex, and ancestry of those 14 individuals.

“The scientific data we provide do not always align with common assumptions,” study co-author and Harvard University geneticist David Reich said in a statement. “For instance, one notable example is the discovery that an adult wearing a golden bracelet and holding a child, traditionally interpreted as a mother and child, were an unrelated adult male and child. Similarly, a pair of individuals thought to be sisters, or mother and daughter, were found to include at least one genetic male. These findings challenge traditional gender and familial assumptions.”


[Related: ‘Violent’ earthquakes accompanied the infamous volcanic eruption that buried Pompeii.]


The genetic data revealed that these Pompeiians had diverse genomic backgrounds. They were primarily descended from a group of eastern Mediterranean immigrants. According to the team, this emphasizes the cosmopolitan nature of the Roman Empire. The findings also highlight the need for using genetic analysis and tools from other scientific disciplines to fully understand the past of Pompeii and beyond.


“Our findings have significant implications for the interpretation of archaeological data and the understanding of ancient societies,” study co-author and Harvard University archaeogeneticist Alyssa Mittnik said in a statement. “They highlight the importance of integrating genetic data with archaeological and historical information to avoid misinterpretations based on modern assumptions. This study also underscores the diverse and cosmopolitan nature of Pompeii’s population, reflecting broader patterns of mobility and cultural exchange in the Roman Empire.”


 
Very interesting publication..
D. Reich is in the list of the authors and we are expecting to see the new data on David Reich Lab site.
It's not there yet. It seems they have some challentes to interpretate their data.. So it will be very interesting to see this data.
 
"For each individual from the casts, ancestry related to Anatolia Neolithic farmers (TUR_Marmara_Barcin_N) and/or Levantine Pre-Pottery Neolithic farmers (Levant_PPN) composes the largest inferred proportion (48%–75%), whereas the second-largest proportion is inferred to derive from people related to Neolithic farmers from Iran (IRN_Ganj-Dareh_N) (26%–45%)."
 
Sample bias as most of those left behind were slaves? Could this be a possibility?

Also, how large was the sample from the city of Rome? Wouldn't indigenous Romans burn their dead?
 
There is no access (bummer)
 
Haplogroups:


Code:
Genetic ID    Y haplogroup call    Excluded lineages
I3686    J-L25    J-Z33968,J-FGC52112,J-FT133466,J-FGC4975
I3685    T-Y4964    T-Y86494,T-CTS9882,T-Y102466,T-Y161528,T-Y39052,T-Y23343,T-Y48600,T-FT4925,T-Y21004,T-CTS6280
I3682    E-Y10561   
I3690    J-L283    J-Z1043,J-Y85328,J-Y15058
I3691    E-L19    E-M81,E-Y141598,E-PF2440

Foreign migrants to the region within the Roman Empire:
We find that the five individuals are shifted away from modern-
day Italians as well as Italian populations from the IA and Late
IA and Imperial-period Etruscans on a principal component anal-
ysis (PCA) constructed on modern-day West Eurasian and
North African populations and world-wide populations. Instead,
they cluster more with eastern Mediterranean, Levantine, and
North African Jewish populations
(Figures 2A and 2B). This
pattern is similar to the one found for the Imperial Roman popu-
lation of Central Italy19 and the previously published single
individual from Pompei
with genome-wide data, which we
co-plotted with the five individuals with newly generated
data. 18

 
Haplogroups:


Code:
Genetic ID    Y haplogroup call    Excluded lineages
I3686    J-L25    J-Z33968,J-FGC52112,J-FT133466,J-FGC4975
I3685    T-Y4964    T-Y86494,T-CTS9882,T-Y102466,T-Y161528,T-Y39052,T-Y23343,T-Y48600,T-FT4925,T-Y21004,T-CTS6280
I3682    E-Y10561
I3690    J-L283    J-Z1043,J-Y85328,J-Y15058
I3691    E-L19    E-M81,E-Y141598,E-PF2440

Foreign migrants to the region within the Roman Empire:




Thanks riverman and tautalus 👍
Riverman Where did you saw the y calls?
(I3691 ) individual is only e1b1b in the pdf
Tautalus shared
so where is the y calls that show he is positive for e-m81 snp ?

Regards
Adam


P.s
Nice to see e-L19 ( I3682 )individual
If real an amazing find
 
Thanks riverman and tautalus 👍
Riverman Where did you saw the y calls?
(I3691 ) individual is only e1b1b in the pdf
Tautalus shared
so where is the y calls that show he is positive for e-m81 snp ?

Regards
Adam


P.s
Nice to see e-L19 ( I3682 )individual
If real an amazing find

That's in the spreadshit S1, slide C.

Dataset S1 C. Y-chromosomal haplogroup assignments.
 
It is already known to archeologists that Pompeii was a city reserved for merchants. It is hardly a representation of the average population of Italy.
I agree
Just cool results 😎
Personally I liked pompeii thats my best memory
when i visited campania 😉
Do you think they are all levantine merchants
Slaves ?
Or could be that some of them came from anatolia or southern greece ?
 
I agree
Just cool results 😎
Personally I liked pompeii thats my best memorry
when i visited campania 😉
Do you think they are all levantine merchants
Slaves ?
Or could be that some of them came from anatolia or southern greece ?
I did a tour there a couple years ago, and our guide said that the city was mostly Merchants, from the Near east, and places like Greece yes. It was also populated with prostitutes and slaves.
wF0JL3V.jpg


Has there ever even been an analysis done on a rural or mountain community outside of these cosmopolitan places? I think it would show a much different story, and demonstrate that the people there are closer to what ever population was present there prior to the Imperial era.
mP8wDnt.jpg
 
I did a tour there a couple years ago, and our guide said that the city was mostly Merchants, from the Near east, and places like Greece yes. It was also populated with prostitutes and slaves.

Has there ever even been an analysis done on a rural or mountain community outside of these cosmopolitan places? I think it would show a much different story, and demonstrate that the people there are closer to what ever population was present there prior to the Imperial era.

I agree that they will have more local IA ancestry, but I think most will still have that kind of "Imperial" admixture, since it was nearly omnipresent, even in the remote provinces. Therefore its extremely unlikely, that it wasn't there in Italia.
 
Don't fool ourselves, those are Indo-European Italic people, not slaves or merchants.
 
I agree that they will have more local IA ancestry, but I think most will still have that kind of "Imperial" admixture, since it was nearly omnipresent, even in the remote provinces. Therefore its extremely unlikely, that it wasn't there in Italia.


Will be interesting to see the y terminal branches
When the bam files will be out there

P.s
Also there autosomal genomes
Should be mostly on the imperial roman profile
But i could be wrong here

By the way I3691 is according to the spreadshit riverman gave link for 👍
is positive of
e-L19 And
e-m81 is excluded for him meaning I3691 was e-L19( x m81)
Interesting I first read it incorrectly
So both I3682 and I3691 are under E-L19 downstreams
Will be smarter when bam files will be out
 
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I rather think that a couple of them will represent (one of) the source(s) of the Imperial Roman Levantine shift, rather than the Imperial Roman average. This being also suggested by not a single haplogroup being local and only one can be considered European (J-L283) from the Balkans.
 
I agree that they will have more local IA ancestry, but I think most will still have that kind of "Imperial" admixture, since it was nearly omnipresent, even in the remote provinces. Therefore its extremely unlikely, that it wasn't there in Italia.
It is not the point though is it? Even if there was, what Jovialis stated is that the people there are closer to what ever population was present in the region prior to the Imperial era (probably an Italic + Aegean mix). Which would easily demonstrate that those Pompeiians are basically newcomers.
 
Pompeii, Stabiae, Herculaneum, and neighboring towns served as favored year-round retreats for Roman aristocrats. The region's delightful climate enticed emperors like Augustus and Tiberius to construct luxurious villas along the picturesque coasts of nearby islands, including the stunning Capri.The presence of the native Roman wealthy elite in Pompeii contributed significantly to the city's diverse population of merchants and slaves. This raised the possibility of discovering the remains of Roman patricians or influential members of society among the human remains, as they were typically cremated. A notable example is Pliny the Elder, who perished in Pompeii; he belonged to the Equestrian Class, a legionary cavalry that was exclusively composed of patricians.
 
A notable example is Pliny the Elder, who perished in Pompeii; he belonged to the Equestrian Class, a legionary cavalry that was exclusively composed of patricians.
Correct and he was from Como, in today's Lombardy. It would be cool to stumble on his remains to see what his autosomal results are, arguably quite different than those found in this study anyway.
 
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